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. 2020 Jul 1;9(6):bio050575. doi: 10.1242/bio.050575

Fig. 3.

Fig. 3.

Effect of Wnt7a overexpression and irradiation on the phosphorylation of JNK, β-catenin in H1650 and A549 NSCLC cell lines. The expression of p-JNK (Thr183/Try185), JNK (total), β-catenin (Thr41/Ser45), β-catenin (Ser552), β-catenin (Ser675), β-catenin (Ser45) and β-catenin (total) were determined by western blot analysis. Representative immunoblots by transfection with Wnt7a and/or irradiation (4 Gy). The phosphorylation level of JNK was increased with the combination of Wnt7a overexpression and irradiation than with Wnt7a overexpression or irradiation alone. GAPDH was used as a loading control.