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. 2020 Jul 1;9(6):bio050575. doi: 10.1242/bio.050575

Fig. 5.

Fig. 5.

The combination of Wnt7a overexpression and irradiation induced the apoptosis in H1650 and A549 NSCLC cell lines through the mitochondrial pathway. The expression of PARP, caspase-3, caspase-7, caspase-9, Bcl-2, Bax and cytochrome c were determined by western blot analysis. Representative immunoblots by transfection with Wnt7a and/or irradiation (4 Gy). The expression of PARP, caspase-3, caspase-7, caspase-9, Bax and cytochrome c were more prominent with the combination of Wnt7a and irradiation than with Wnt7a overexpression or irradiation alone. The expression of Bcl-2 was suppressed with the combination of Wnt7a and irradiation than with Wnt7a overexpression or irradiation alone. GAPDH was used as a loading control.