Fig. 1.

Amplification, DNA cloning and positive clone identification from ramp-PCR amplified fragments. a The ramp-PCR amplification from DNA samples of Lycium species. Lanes 1–16 are different Lycium species samples listed in Table 1 (Liu et al. 2020). The blue arrow in lanes 2 and 16 is indicated bands for cutting. b The quality and quantity checking of ramp-PCR fragments purified from an agarose gel. The blue arrows indicate a fragment named “10” which we cut different PCR bands and a band of T vector, respectively. c Identification of positive clone by plasmid DNA digestion. Lane 1 indicates the DNA molecular weight marker DL2000 with the fragment sizes (bp) 2000, 1000, 750, 500, 250, 100. Lanes 2 and 3 indicate clone 10–5 plasmid DNA without (−) or with (+) EcoR I digestion. The blue arrow indicates expected insert of RAPD DNA fragment