Fig. 3.

Development of RAPD-SCAR marker Goji 10–5. a Development of RAPD-SCAR marker Goji 10–5. Lanes 1–16 indicate the different Lycium species samples listed in Table 1 described in Material and Method and the previously published paper (Liu et al. 2020). Lane 17 is PCR amplification without any DNA samples as a negative control. b Discriminating Nitraria sibirica Pall. from Goji samples by Goji 10–5 marker. Lanes 1–4 indicate a negative control without any DNA samples, two samples from Jiangxi in China and Houston, Texas, in the USA, and a N. sibirica Pall. sample, respectively (b). No any specific PCR band is found in the lane 4 of N. sibirica Pall. sample DNA. Lane M indicates the DNA molecular weight marker DL2000 with the indicated fragment sizes