TABLE 1.

List of selected Web-sites for gRNA design in multi-species.

Name	Link	PAM	Organism	Function	References
CHOPCHOP v3	http://chopchop.cbu.uib.no	Most reported PAMs or a self-defined sequence	Over 200 genomes	Knock out/knock in/activation/repression/Nanopore enrichment	Labun et al., 2019
E-CRISPR	http://www.e-crisp.org/	Most reported PAMs	Over 50 genomes	Single design/paired designs	Heigwer et al., 2014
ATUM	https://www.atum.bio/eCommerce/cas9/input	NGG/NAG	Homo sapiens/Mus musculus/Saccharomyces cerevisiae/Escherichia coli/Arabidopsis thaliana
CRISPRdirect	https://crispr.dbcls.jp/	Self-defined PAM	Over 200 species		Naito et al., 2015
CRISPR-ERA	http://crisprera.stanford.edu/	NGG	Human/mouse/rat/zebrafish/ D. melanogaster/ C. elegans/S. cerevisiae/ E. coli/B. subtilis	Gene editing/activation/repression	Liu et al., 2015
CC TOP	https://crispr.cos.uni-heidelberg.de	Most reported PAMs	102 species	gRNA and off-target prediction	Stemmer et al., 2015