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Immunization protocol in experimental and control groups.
| Groups | 1st (0 Day) | 2nd (14 Days) | 3rd (28 Days) |
| Blank control | No immunity | No immunity | No immunity |
| Adjuvant control (μl) | 200 | 200 | 200 |
| pET-32a protein control (μg) | 100 | 100 | 100 |
| Recombinant TvAP33 protein (μg) | 100 | 100 | 100 |
