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. 2020 Jun 30;8:695. doi: 10.3389/fbioe.2020.00695

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Copyright © 2020 Xia, Li, Zhong and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Top: composition of the three-enzyme–ZIF-8 NMOF hybrid. Bottom: scheme of the three-enzyme cascades involving β-Gal hydrolysis of lactose and subsequent GOx-catalyzed oxidation of glucose, followed by HRP-catalyzed oxidation of Amplex Red to resorufin by H₂O₂. (B) Top: composition of the bio-catalytic constituents AlcDH, LacDH and the NAD⁺–polymer in the ZIF-8 NMOF hybrid nanoparticles. Bottom: scheme of the coupled NAD⁺–polymer mediated AlcDH/LacDH-catalyzed reduction of pyruvic acid to lactic acid by the bio-catalytic NMOF hybrid assembly. Reproduced from Chen et al. (2018b) with permission from Nature Publishing Group, copyright 2018.