Table 1.
Primers used in qRT-PCR primer assays.
| Gene symbol | Accession no | Product length | Probe no./SYBR | Sequence 5′–3′ |
|---|---|---|---|---|
| GAPDH | NM_002046.3 | 72 | P3 | CAGCAAGAGCACAAGAGGAA |
| GTGGTGGGGGACTGAGTGT | ||||
| KIT | NM_000222.2 | 61 | P6 | GAGTAGCTTACCAGAAGCTTCCATAG |
| CATAGGGACTGATGCCTTCC | ||||
| KRT3 | NM_057088.2 | 92 | P74 | CCTGTGATTGTCCAGGTGTG |
| ATACATCAGAGCTGTAGTGAGCATC | ||||
| KRT15 | NM_002275.3 | 60 | P10 | CATTGGCATCAGGGAAGC |
| TTGATGTGGAAATTGCTGCT | ||||
| LAMA1 | NM_005559.3 | 60 | P38 | AGGATGACCTCCATTCTGACTT |
| CCTTACATGGGCACTGACCT | ||||
| LAMA2 | NM_000426.3 | 68 | P81 | GCAAGCCACTGGAGGTTAAT |
| TGGGCATGATACAGGTTGAA | ||||
| LAMA3 | NM_198129.1 | 65 | P29 | CCAGGAATATGGGTTGCTTG |
| GGGAGCAGCACCAGGTAAT | ||||
| LAMA4 | NM_001105209.1 | 68 | P55 | GCCACACTCGTCCTTCTCTC |
| CCCAGGTGAAACTCTCAAGG | ||||
| LAMA5 | NM_005560.3 | 75 | P44 | ATGACTCGCTCTGTGGAGGT |
| GGGGTTGGCTGTGTCCTA | ||||
| LAMB1 | NM_002291.2 | 72 | P82 | AAGCCAGAAAGTTGCTGTGTATAG |
| CCTTGTTCACCTCAGCCATT | ||||
| LAMB2 | NM_002292.3 | 100 | P43 | AAGGCCTACCCCAGTTCCTA |
| GGGTTCACACTGGTTTATTGG | ||||
| LAMB3 | NM_001127641.1 | 72 | P23 | GGCATGCCATTGAAACTAAGA |
| AGAACTAAAGGCGGGGGATA | ||||
| LAMB4 | NM_007356.2 | 67 | P5 | CCCCACACCCTGTCCTTATT |
| ATTTTCCTGGTGGCATTTCA | ||||
| LAMC1 | NM_002293.3 | 92 | P66 | GCCATTATTTTATTGTCTAGCTCCA |
| ATCCCTGTGTCAACCAGCAT | ||||
| LAMC2 | NM_005562.2 | 96 | P65 | CACTCTGTGCCTTTCTACAACTG |
| CCAAGGTGGAAGTGCCTCT | ||||
| LAMC3 | NM_006059.3 | 67 | P14 | CAGGACTCCTCAGCATTTCC |
| TTGCCATCTGCTGGAAGAG | ||||
| MLANA | NM_005511.1 | 79 | P39 | GAGAAAAACTGTGAACCTGTGGT |
| AAGGTGGTGGTGACTGTTCTG | ||||
| NT5E | NM_002526.4 | 111 | P80 | CCAGTCCACTGGAGAGTTCC |
| CGACACTTGGTGCAAAGAAC | ||||
| TYRP1 | NM_000550.2 | 77 | P2 | CCTGTGACCAGAGGGTTCTC |
| CCGGACAAAGTGGTTCTTTTC |
Primers were used for Probe based (Universal Probe Library) or SYBR Green based qRT-PCR assays with an annealing temperature of 60 °C.