Table 2.
List of antibodies used.
| Antibody (clone), Host species | Antibody concentration | Application | Antibody source |
|---|---|---|---|
| CD49c (Integrin α3) APC (P1B5), mouse | 1.25 µg/ml | Flow cytometry | eBioscience |
| CD49f (Integrin α6) APC (GoH3), rat | 1.25 µg/ml | Flow cytometry | eBioscience |
| CD29 (Integrin β1) FITC (TS2/16), mouse | 2.5 µg/ml | Flow Cytometry | eBioscience |
| CD104 (Integrin β4) eFluor 660 (439-9B), rat | 10 µg/ml | Flow cytometry | eBioscience |
| CD54 (ICAM-1) APC (HA58), mouse | 10 µg/ml | Flow cytometry | BD |
| CD117 (c-Kit) APC (YB5.B8), mouse | 10 µg/ml | Flow cytometry | BD |
| IgG1 isotype PE (MOPC-21), mouse | 5 µg/ml | Flow cytometry | BD |
| IgG1 isotype FITC (IS5-21F5), mouse | 2.5 µg/ml | Flow cytometry | Miltenyi Biotec |
| IgG1 isotype APC (IS5-21F5), mouse | 1.25–2.5 µg/ml | Flow cytometry | Miltenyi Biotec |
| IgG2b isotype APC (141945), rat | 1.25–10 µg/ml | Flow cytometry | R&D systems |
| Melan-A PE (M2-7C10 + M2-9E3), mouse | 10 µg/ml | Flow cytometry | Novus biologicals |
| Integrin α3 (ASC-1), mouse | 20 µg/ml | Blocking | Millipore |
| Integrin α6 (NKI-GoH3), rat | 20 µg/ml | Blocking | Millipore |
| Integrin β1 (P5D2), mouse | 2.5 µg/ml | Blocking | R&D systems |
| CD117/c-Kit (Ab81), mouse | 1:500 | Immunohisto/cytochemistry | Cell signaling |
| Cytokeratin pan (AE1/AE3), mouse | 1:100 | Immunohistochemistry | Dako |
| Cytokeratin 15 (LHK15), mouse | 1:500 | Immunohisto/cytochemistry | Abcam |
| HMB-45 (HMB45), mouse | 1:50 | Immunohisto/cytochemistry | Dako |
| Integrin α3 (P1B5), mouse | 1:200 | Immunohistochemistry | Dako |
| Integrin α6 (GoH3), rat | 1:100 | Immunohistochemistry | Chemicon/Millipore |
| Integrin β1 (HB1.1), mouse | 1:500 | Immunohistochemistry | Chemicon/Millipore |
| Integrin β4 (439-9B), rat | 1:200 | Immunohistochemistry | Chemicon/Millipore |
| Ki-67 (SP6), rabbit | 1:1,000 | Immunocytochemistry | Abcam |
| Laminin α1 (317), rabbit | 1:500 | Immunohistochemistry | L. Sorokin |
| Laminin α2 (401), rabbit | 1:500 | Immunohistochemistry | L. Sorokin |
| Laminin α3, rabbit | 1:6,000 | Immunohistochemistry | R. Timpl/T. Sasaki |
| Laminin α5 (405), rabbit | 1:4,000 | Immunohistochemistry | L. Sorokin |
| Laminin β1 (IIID9), mouse | Undiluted | Immunohistochemistry | L. Sorokin |
| Laminin β2 (409), rabbit | 1:200 | Immunohistochemistry | L. Sorokin |
| Laminin β3, rabbit | 1:6,000 | Immunohistochemistry | R. Timpl/T. Sasaki |
| Laminin γ1 (3E10), rat | undiluted | Immunohistochemistry | L. Sorokin |
| Laminin γ2 (LE4-6), rabbit | 1:4,000 | Immunohistochemistry | R. Timpl/T. Sasaki |
| Laminin γ3, rabbit | 1:2000 | Immunohistochemistry | R. Timpl/T. Sasaki |
| Melan-A (A103), mouse | 1:25 | Immunohisto/cytochemistry | Dako |
| Melan A, (EP1422Y), rabbit | 1:500 | Immunohisto/cytochemistry | Abcam |
| MITF (C5), mouse | 1:500 | Immunohisto/cytochemistry | Abcam |
| Nestin (10C2), mouse | 1:100 | Immunohisto/cytochemistry | Abcam |
| Sox10 (BC34), mouse | 1:50 | Immunohisto/cytochemistry | Abcam |
| TRP1 (TA99), mouse | 1:100 | Immunohisto/cytochemistry | Abcam |
| Vimentin, (280618), rat | 1:50 | Immunohisto/cytochemistry | R&D Systems |