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. 2020 Jul 6;10:11063. doi: 10.1038/s41598-020-67528-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of the top 25 abundant bacteria at the phylum level and their cluster analysis under different treatments as visualised by heatmaps (variables clustering on the vertical axis). CK: untreated soil; B: soil treated with biochar; B + U: soil treated with biochar and urea; Urea: soil treated with only urea as nitrogen fertilizer; BCRNF: soil treated with BCRNF as nitrogen fertilizer. The colour intensity of the scale indicates the relative abundance of each phylum. Relative abundance is defined as the number of sequences affiliated with that taxon divided by the total number of sequences per sample (%).