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. 2020 Jul 6;10:11118. doi: 10.1038/s41598-020-68022-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) rNOE time course of the effect of the heat shock on collagenized MCAs in medium. The dark grey shaded background indicates the heat shock (HS) of 42 °C, while the two light grey shaded backgrounds indicate the heat up (HU) and cool down (CD) to 37 °C. (c) Temporal stability of the rNOE signal from a cell culture during dynamic measurements but without heat shock application. The rNOE signal was constant over time, which was also confirmed by similar AREX spectra before and after dynamic measurements acquired at time 0 and 295 min respectively, as shown in (b) and (d).