Figure 3.

(a) Time course of the rNOE signal from cell culture 1 during dynamic measurements. The heat shock (HS) of 42 °C is indicated by the dark grey shaded background, while the two light grey shaded backgrounds close by indicate the heat up (HU) to 42 °C and the cool down (CD) to 37 °C. Below the time course, normalized AREX_ΔST images of the cell area overlaid on a ¹H RARE image are shown. After the heat shock, a substantial rNOE signal reduction of 8.3 ± 1.1% followed by an exponential recovery with a recovery time of 100.0 ± 52.5 min was observed. An rNOE signal recovery to the initial value was also confirmed by the AREX spectra before and after dynamic measurements acquired at time 0 and 295 min, respectively, which are shown in (b).