Figure 5.

(a) Graphical sketch of the bioreactor setup. A peristaltic pump supplied cells at 400 µl/min with fresh medium, which was aerated by a gas mixing station. A temperature of 37 °C inside the bioreactor was achieved by heating the animal bead and the tubes prior to the bioreactor by the first heat bath. To achieve a heat shock of 42 °C, a second heat bath was added to the bioreactor setup. (b) (i) Dimension of bioreactor, (ii) bioreactor cross-section with perfusion of the medium, (iii) ¹H RARE image of the bioreactor loaded with two MCAs and (iv) light microscope image of one MCA are shown. Fresh medium entered the bioreactor at the bottom, which was then perfused through the pores of the MCAs and subsequently left the bioreactor on top. The size of the cavity area is 1 × 1 cm², while each cavity has a depth and diameter of 300 µm.