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. 2020 Jul 6;11(7):506. doi: 10.1038/s41419-020-2701-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a PD-L1 was knocked down by siRNA in H460 and H358 cells. (b) Overlap of changed genes affected by PD-L1 siRNA silencing in H460 and H358 lung cancer cells. c Heatmap showing the subset of 12 genes identified in H460 and H358 NSCLC cells followed by PD-L1 silencing. d The regulation of gene expression by PD-L1 was analyzed by RT-PCR assay in H460 and H358 cells. e KEGG analysis showed that genes were enriched in the actin cytoskeleton pathway. f, g The expression of WIP was detected by RT-PCR and western blot.