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. 2020 Jun 30;11:1361. doi: 10.3389/fimmu.2020.01361

Figure 7.

Figure 7

The protective effects of C-L on the intestinal barrier. (A) TEER values in mouse jejunum epithelium were detected using a Ussing chamber. (B) Expression levels of epithelial barrier function-related proteins (occluding and ZO-1) were determined by western blotting. (C) For immunofluorescence staining of occludin (red color) in jejunum tissue, stained slides with the (C-a) Control, (C-b) EHEC, (C-c) EHEC+Enro, (C-d) EHEC+C-LL, (C-e) EHEC+C-LM, (C-f) EHEC+C-LH groups were observed under a fluorescence microscope. Scale bar, 100 μm. (D) The protective effects of C-L on intestinal tight junction structures were examined by TEM for the (D-a) Control, (D-b) EHEC, (D-c) EHEC+Enro, (D-d) EHEC+C-LL, (D-e) EHEC+C-LM, (D-f) EHEC+C-LH groups. Narrower intervals and clearer desmosomes (black arrows) between the intestinal epithelial cells were found in C-L-treated mice. Scale bar, 2 μm. The control group was orally administered 100 μL sterile PBS; the EHEC group was orally administered 100 μL sterile PBS containing 1 × 108 CFUs EHEC O157:H7; the EHEC+Enro group was orally administered 100 μL sterile PBS containing 1 × 108 CFUs EHEC O157:H7 and then treated by i.p. injection with 8 mg/kg Enro once/day for 3 days; the EHEC+C-LL, EHEC+C-LM, and EHEC+C-LH groups were administered 100 μL sterile PBS containing 1 × 108 CFUs EHEC O157:H7 and then treated by i.p. injection with 4, 8, and 16 mg/kg C-L, respectively, once/day for 3 days. The data are shown as the mean ± standard deviation (n = 5). NS, P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.