Figure 5.

NCz-SEGN24A+CpG, but not its individual antigens, confers protection against T. cruzi infection. Immunized mice with NCz-SEGN24A+CpG, individual antigens Nt-Cz+SEGN24A+CpG or saline (n = 5/group) were challenged intraperitoneally 15 days after the last dose with 1 × 10⁴ trypomastigotes from the RA strain. (A) Parasitemia levels were monitored during the acute phase of the infection in 5 μl of blood taken every 2 days. (B) AUC of the parasitemia curve. (C) Survival rates were monitored daily over an extended period of the acute phase. Data are expressed as the mean ± SEM of three independent experiments. Asterisks represent statistical significance respect to saline group. *p < 0.05. One-way ANOVA plus Tukey's post-test (B).