Skip to main content
. 2020 Jul 6;6:56. doi: 10.1038/s41420-020-0290-3

Fig. 4. TGR5-mediated bile acids induced HNF4α and columnar genes expression.

Fig. 4

a GES-1 and HFE-145 cells were treated with SB756050 (2, 5, and 10 μM) for 24 h. Then, HNF4α protein expression was detected by western blotting (WB). b GES-1 cells were transfected with TGR5 overexpression lentivirus. HNF4α mRNA expression were examined by qRT-PCR. Error bar indicates the SEM, **P < 0.01 vs. negative control (NC), n = 3. TGR5, p-ERK1/2, and HNF4α protein expression were examined by WB. c AGS cells were transiently transfected with siRNA against TGR5. TGR5 and HNF4α mRNA was examined by qRT-PCR. Error bar indicates the SEM, **P < 0.01 vs. NC, n = 3. HNF4α protein expression was examined by WB.