Fig. 7. HNF4α-mediated bile acids induced columnar genes expression.

a, b BGC823 cells (a) and AGS cells (b) were treated with different doses of BI6015 for 24 h. CDX2, KLF4, Villin1, CDH17, and ALPI expression was examined by western blot (WB). c GES-1 cells were pretreated with BI6015 (2, 5, and 10 μM) for 1 h and then treated with DCA (200 μM) for another 24 h. KLF4, CDX2, ALPI, and Villin1 expression was detected by WB. d HNF4α and CDX2 were knocked down by stable transfection with shRNA lentiviral in GES-1 cells. Next, the cells were treated with DCA (200 μM) for 24 h. HNF4α, CDX2, KLF4, and p21 were examined by WB.