Table 2.
Primers used in the study for reverse transcription quantitative PCR (RT-qPCR).
| Gene symbol |
GenBank access number (species) |
Primer sequences [forward (F) and reverse (R)] |
Amplicon size (bp) | Refs |
|---|---|---|---|---|
| Reference genes | ||||
| ATP1A1 |
(Ovis aries) |
F: GCA GGG GAT GAA GAA CAA GA R: GAG AAG CGA GTA GGG GAA GG |
154 | 12 |
| RPL19 |
gi94966830 (Bos taurus) |
F: ATG AAA TCG CCA ATG CCA AC R: GGC AGT ACC CTT TCG CTT ACC |
167 | 51 |
| UBB | NM_001009202.1 (Ovis aries) |
F: GCA TTG TTG GGT TCC TGT GTC R: CAC GAA GAT TTG CAT TTT GAC |
98 | 51 |
| Target gene | ||||
| CDK9 | XM_012147609.2 (Ovis aries musimon) |
F: GCA AAG CAG TAC GAC TCG GT R: GCC TTA AAC ACC TCC CCG AA |
104 | This study |
| CMYC | NM_001046074.2 (Bos taurus) |
F: CCA GTA GCG ACT CTG AGG AAG R: TGT GAG GAG GTT TGC TGT GG |
135 | This study |
| RONIN (THAP11) |
(Bos taurus) |
F: CAC GGG AGA AGA CGT TAA GC R: GGA GCC AGT ATC AGG GAA GC |
187 | 51 |
| RXRβ |
(Bos taurus) |
F: TGG GAG CCA TCT TCG ATA GGG T R: CCT TGG CAT CTG GAT TGA ACA GAA |
120 | This study |
| TBX3 |
(Ovis aries) |
F: CTG CTA CTG GGG AAC AGT GG R: GGG AAG GCC AAA GTA AAT CCG |
100 | This study |
| TCF3 |
(Bos taurus) |
F: GTG AGA AGC CCC AGA CCA AA R: GGG TTC AGG TTA CGC TCT CG |
94 | This study |
Refs: references.