Fig. 1. Generation of inducible BRN2-expressing human melanoma cell lines.

a Production of cells with doxycycline-inducible induction of BRN2 or lacZ in melanoma cell lines. Western blot analysis was performed on MM370, MM455, and MM603 cells, 48 h after induction of expression by exposure to 50 ng/ml doxycycline. * long exposure for MITF in MM455 is also shown. b MM370, MM455, or MM603 cells transduced to express BRN2 (circles) or lacZ (squares) were seeded into 96-well plates and treated with doxycycline (open symbols) or vehicle (closed symbols) for 7 days. Sulforhodamine B assay was used to measure cellular proliferation rate. Values indicate mean ± SD; n = 3 independent experiments with at least triplicate readings.