Fig. 4. Identification of novel BRN2 target genes.

a Comparison of upregulated genes following BRN2 re-expression with previously published BRN2 ChIP data²⁴. b Comparison of downregulated genes following BRN2 re-expression with BRN2 ChIP data. c, d Chromatin immunoprecipitation (ChIP) assays from c MM370 or d MM455 cells were performed as described in the Materials and Methods. qPCR was used to determine the percentage of total chromatin input immunoprecipitated by the anti-BRN2 antibody. Values indicate mean ± SD, n = triplicate values from three independent experiments. e Protein lysates from SK-MEL-28 or HT144 melanoma cell lines treated with 50 nm validated siRNA targeting MITF, BRN2, or GFP as a control (NEG) were examined by western blot analysis. The blots were probed with MITF antibody followed by BRN2, c-MET, and GAPDH antibodies.