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. 2020 Jul 6;9(7):64. doi: 10.1038/s41389-020-00247-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Cellular viability counts following 7 days of growth under ultra-low-adherence conditions either with or without induction of expression of BRN2 or lacZ as a control. Values indicate mean ± SEM, n = at least duplicate counts from three independent experiments. **p < 0.01; ****p < 0.0001; unpaired t test. b, c Protein lysates from melanoma cell lines treated with 50 ng/ml doxycycline or vehicle to induce expression of BRN2 or lacZ as a control for 7 days in ultra-low-adherence conditions were examined by western blot analysis. n.d. – not detected. d Protein lysates from melanoma cell lines grown under adherent conditions with BRN2 or lacZ induction for 2 days. The blots were probed with phospho-STAT3 antibody followed by total STAT3, BRN2, MITF (where indicated) and GAPDH antibodies. Representative blots of two independent experiments are shown.