Figure 4.

CD49c/CD29 play a role in the galectin-3 mediated inhibition of γδ T cell proliferation within PBMC. (A,B) In total, 5 × 10³ PancTu-I cells were seeded for 24 h. 2.5 × 10⁵ PBMC/well were pre-incubated with (A) the indicated concentrations of the displayed antibodies or appropriate isotype controls or (B) with 10 μg/mL anti-CD29 mAb together with 10 μg/mL anti-CD49c mAb or appropriated isotype controls for 2 h. (A,B) Thereafter, PancTu-I cells were transferred and stimulated with 2.5 μM zoledronic acid and 50 IU/mL rIL- 2. After 7 days, the absolute cell number of Vγ9 γδ T cells was determined using SCDA. (A) The means ± SD of duplicates of 2 donors are shown. (B) Each point represents an individual donor. Statistical comparison of matched samples was carried out parametrically by using paired, two-tailed t-test. P-value; *P < 0.05.