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. 2020 Jun 30;11:1481. doi: 10.3389/fmicb.2020.01481

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Copyright © 2020 Zhang, Zhu, Yu, You, Wang, Cao, Liu, Wang, Kong, Toufeeq and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of virus-binding in the microsomal proteins fraction of the P50 midgut on SDS-PAGE. (A) Separation of Bombyx mori midgut microsomes (MS) and BmNPV budded viruses (BVs) proteins by SDS-PAGE. (B) Virus overlay binding experiment. Microsomes protein blots, overlaid with (MS + BV) and without BVs (MS-BV) were incubated with antibodies against the AcMNPV gp64 protein to detect the BVs. BmNPV Budded virus was used as a positive control (BV) to gp64 protein. Arrows d and e in (A,B) refer to the bands detected in the stained gel and the PVDF membrane. M, protein molecular weight marker.