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. 2020 Jun 30;8:426. doi: 10.3389/fcell.2020.00426

FIGURE 1.

FIGURE 1

Murine embryo development is impacted by DMP, BPA, DEP and DEHP. In vitro embryo development on day 5 post fertilization. Panels (A,C,E,G) are representative images of blastocysts previously exposed at the zygote stage to 0, 1, or 10 μM of the indicated EDC for 20 h. The subsequent embryo culture was done in the absences of the indicated EDC. (A) Shown are representative images of DMP-exposed embryos. (B) The survival rate of DMP exposed zygotes was calculated based on the percentage of embryos that have reached the morula or blastocyst stage. (C) Representative images of embryos previously exposed to BPA. (D) The survival rate of BPA exposed zygotes was calculated as in (B). (E) Representative images of embryos previously exposed to DEP. (F) The survival rate of DEP exposed zygotes was calculated as in (B). (G) Representative images of embryos previously exposed to DEHP. (H) The survival rate of DEHP- exposed zygotes was calculated as in (B). Data are means ± S.E.M. Asterisk indicates a statistical difference between control embryos and embryos exposed to EDCs. *P ≤ 0.05, **P < 0.01, ***P < 0.001. Scale bars for all images are 50 μm.