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. 2020 Jun 30;8:426. doi: 10.3389/fcell.2020.00426

FIGURE 2.

FIGURE 2

Fertilization rate of murine eggs exposed to EDCs treated spermatozoa. Panels (A,C,E,G) are representative images of blastocysts obtained after murine eggs were introduced to sperm previously exposed to 0, 1, or 10 μM of the indicated EDC. Subsequent embryo culturing was done in the absences of EDCs, and the images were taken on day 5 post insemination. (A) Shown are representative images of blastocysts obtained after murine eggs were introduced to sperm previously exposed to DMP. (B) Percentage of eggs that were fertilized by DMP- treated sperm and were able to reach morula or blastocyst stage. (C) Representative images of embryos obtained after IVF with BPA-treated spermatozoa. (D) The percentage of eggs fertilized by BPA-treated sperm was calculated as in (B). (E) Representative images of embryos obtained after IVF with DEP-treated sperm. (F) The percentage of eggs fertilized by DEP-treated sperm was calculated as in (B). (G) Representative images of embryos obtained after IVF with DEHP-treated sperm. (H) The percentage of eggs fertilized by DEHP-treated sperm was calculated as in (B). Data are means ± S.E.M. Asterisk indicates a statistical difference between control embryos and embryos exposed to EDCs. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars for all images are 50 μm.