Fig. 2.

Mutations at serine phosphorylation site do not alter APJ receptor expression or localization to the cell membrane.

A, Cell surface expression of WT and APJ mutants (S335A, S339A, S345A), quantified by ELISA. The results are expressed as the percentage of WT APJ cell surface expression, after correction for nonspecific expression in cells transfected with an empty vector (n = 5; one-way analysis of variance; ns, no significant difference, P > 0.05). B, BRET data indicating proximity between the cell surface marker Venus-Kras and Rluc-tagged WT or mutant receptors (S335A, S339A, S345A) (n = 5; one-way analysis of variance; ns, no significant difference, P > 0.05).