Figure 2.

Arabidopsis and tomato mutants with decreased flavonoid antioxidants have increased ROS accumulation. (A) Confocal micrographs of DCF-stained guard cells of 4-week-old VF36 (wild-type) and are plants show that flavonol deficient mutants have increased ROS levels both in the absence or presence of 20 µM ABA. (B) Confocal micrographs of DCF and PO1 fluorescence in guard cells of 4-week-old tomato and Arabidopsis leaves show tomato and Arabidopsis with decreased flavonol levels have increased total ROS and H₂O₂ levels. Scale bars = 5 µm. DCF signal is shown in green, PO1 signal in blue, and chlorophyll autofluorescence in magenta. Images obtained from experiments completed in (Watkins et al., 2014; Watkins et al., 2017).