Table 3.
Goodness-of-fit test results for a simple linear regression between the immunopathological outcomes of fish challenged by R. salmoninarum at different water temperatures.
| Biomarker group | Abbreviation | Biomarker | Df | SS | MS | F-value | Pr(>F) | R2 | R-adjusted |
|---|---|---|---|---|---|---|---|---|---|
| Microscopic lesions | hsBKD | Histoscore BKD | 1 | 0.26927 | 0.26927 | 55.886 | 0.00000 | 0.381 | 0.374 |
| Plasma enzymes. substrates and minerals profile | Sodium (mmol/L) | NA | 1 | 1642.8 | 1642.82 | 29.712 | 0.00000 | 0.287 | 0.277 |
| Chloride (mmol/L) | CL | 1 | 650.5 | 650.47 | 13.414 | 0.00047 | 0.155 | 0.144 | |
| Potassium (mmol/L) | K | 1 | 0.7644 | 0.76439 | 11.268 | 0.00125 | 0.132 | 0.120 | |
| Phosphorus (mmol/L) | P | 1 | 381915 | 381915 | 295.54 | 0.00000 | 0.846 | 0.843 | |
| Iron (mmol/L) | FE | 1 | 577417 | 577417 | 124.04 | 0.00000 | 0.646 | 0.641 | |
| Magnesium (mmol/L) | MG | 1 | 674.67 | 674.67 | 62.068 | 0.00000 | 0.466 | 0.459 | |
| Calcium (mmol/L) | CA | 1 | 1084.9 | 1084.92 | 30.556 | 0.00000 | 0.301 | 0.291 | |
| Alcaline phosphatase (U/L) | ALP | 1 | 331208 | 331208 | 392.22 | 0.00000 | 0.875 | 0.873 | |
| Pancreatic amilase (U/L) | PAM | 1 | −1.2237 | 0.1297 | −9.435 | 0.00000 | 0.655 | 0.647 | |
| Lipase (U/L) | LIP | 1 | −0.23511 | 0.02741 | −8.579 | 0.00000 | 0.610 | 0.602 | |
| Total amilase (U/L) | AMI | 1 | −1.2319 | 0.1492 | −8.259 | 0.00000 | 0.563 | 0.555 | |
| Creatine kinase (U/L) | CK | 1 | 34.354 | 34.354 | 38.502 | 0.00000 | 0.407 | 0.397 | |
| Aspartate aminotransferase (U/L) | AST | 1 | 124928 | 124928 | 12.914 | 0.00065 | 0.175 | 0.161 | |
| Alaline aminotransferase (U/L) | ALT | 1 | 0.2764 | 0.276398 | 2.8226 | 0.09823 | 0.046 | 0.029 | |
| Lactate dehydrogenase (U/L) | LDH | 1 | 0.0884 | 0.08843 | 0.2331 | 0.63150 | 0.005 | −0.016 | |
| Cholesterol (mmol/L) | CHOL | 1 | 979.86 | 979.86 | 391.12 | 0.00000 | 0.827 | 0.825 | |
| Albumin (g/L) | ALB | 1 | 9.096 | 9.096 | 126 | 0.00000 | 0.612 | 0.607 | |
| High density lipoprotein (mmol/L) | HDL | 1 | 133.24 | 133.24 | 94.498 | 0.00000 | 0.578 | 0.572 | |
| Urea (mmol/L) | URE | 1 | 107.378 | 107.378 | 114.53 | 0.00000 | 0.566 | 0.561 | |
| Low density lipoprotein (mmol/L) | LDL | 1 | 22.697 | 22.6965 | 57.944 | 0.00000 | 0.457 | 0.449 | |
| Total protein (g/L) | TPO | 1 | 7.4125 | 7.4125 | 70.242 | 0.00000 | 0.436 | 0.429 | |
| Glucose (mmol/L) | GLU | 1 | 13.328 | 13.3282 | 57.934 | 0.00000 | 0.429 | 0.422 | |
| Globulins (g/L) | GLO | 1 | 5.5818 | 5.5818 | 49.639 | 0.00000 | 0.353 | 0.346 | |
| Lactate (mmol/L) | LAC | 1 | 0.47979 | 0.47979 | 18.456 | 0.00004 | 0.169 | 0.160 | |
| Uric acid (μmol/l) | UAC | 1 | 1191 | 1191.04 | 17.006 | 0.00008 | 0.158 | 0.148 | |
| Creatinine (μmol/l) | CRE | 1 | 1677.4 | 1677.38 | 13.779 | 0.00037 | 0.144 | 0.133 | |
| Triglycerides (mmol/L) | TRG | 1 | 0.0002 | 0.000201 | 0.0046 | 0.94640 | 0.050 | −0.011 | |
| Hematological profile | Hematocrit (%) | HCT | 1 | 11.6577 | 11.6577 | 231.53 | 0.00000 | 0.773 | 0.770 |
| Red blood cell count (x10e6/UI) | RBC | 1 | 6.6981 | 6.6981 | 221.44 | 0.00000 | 0.735 | 0.731 | |
| Hemoglobin (g/L) | HB | 1 | 7.0518 | 7.0518 | 67.127 | 0.00000 | 0.438 | 0.432 | |
| Mean Corpuscular Hemoglobin Concentration (g/L) | MCHC | 1 | 0.37926 | 0.37926 | 14.353 | 0.00028 | 0.143 | 0.133 | |
| Mean Corpuscular Hemoglobin (f/L) | MHC | 1 | 0.0439 | 0.04391 | 0.9586 | 0.33050 | 0.012 | −0.001 | |
| Immature red blood cell (N°/mL) | IRBC | 1 | 20175918 | 20175918 | 67.026 | 0.00000 | 0.626 | 0.617 | |
| Lymphocytes count (N°/mL) | LYM | 1 | 814043587 | 814043587 | 63.828 | 0.00000 | 0.477 | 0.470 | |
| Heterophils count (N°/mL) | HET | 1 | 13530470 | 13530470 | 54.819 | 0.00000 | 0.450 | 0.442 | |
| Monocytes count (N°/mL) | MON | 1 | 124.54 | 124.544 | 34.336 | 0.00000 | 0.389 | 0.377 | |
| White blood cell count (N°/μL) | WBC | 1 | 739782809 | 739782809 | 42.168 | 0.00000 | 0.376 | 0.367 | |
| Blastocytes (N°/mL) | BLA | 1 | 452070 | 452070 | 18.157 | 0.00009 | 0.275 | 0.259 | |
| Thrombocytes or Platelet Count (N°/μL) | PLC | 1 | 2.9866 | 2.98657 | 11.172 | 0.00191 | 0.232 | 0.211 | |
| Unidentified cells (N°/mL) | UIC | 1 | 196 | 195.99 | 1.9546 | 0.17040 | 0.050 | 0.025 | |
| Immunological profile (RT–qPCR) | Cluster of differentiation 8 | CD8 | 1 | 10.9629 | 10.9629 | 155.27 | 0.00000 | 0.680 | 0.676 |
| Eomesodermin | EOMES | 1 | 7.4297 | 7.4297 | 113.36 | 0.00000 | 0.669 | 0.663 | |
| Interleukin 12 | IL-12 | 1 | 6.4925 | 6.4925 | 116.23 | 0.00000 | 0.663 | 0.658 | |
| Interleukin 2 | IL-2 | 1 | 2.561 | 2.56097 | 61.368 | 0.00000 | 0.600 | 0.590 | |
| T-bet | TBET | 1 | 2.9251 | 2.92511 | 87.005 | 0.00000 | 0.596 | 0.589 | |
| Granzyme A | GZMA | 1 | 23.142 | 23.1419 | 49.221 | 0.00000 | 0.468 | 0.458 | |
| Perforin 2 | MPEG | 1 | 2.4807 | 2.48073 | 38.634 | 0.00000 | 0.408 | 0.398 | |
| Interleukin 10 | IL-10 | 1 | 18.428 | 18.4285 | 38.98 | 0.00000 | 0.386 | 0.376 | |
| Immunoglobulin T | IGT | 1 | 6.058 | 6.058 | 33.35 | 0.00000 | 0.282 | 0.273 | |
| GATA-3 | GATA3 | 1 | 3.6406 | 3.6406 | 23.095 | 0.00001 | 0.281 | 0.269 | |
| Interferon gamma | IFNG | 1 | 1.785 | 1.785 | 15.894 | 0.00025 | 0.265 | 0.249 | |
| Interleukin 4/13 | IL4/13 | 1 | 0.85802 | 0.85802 | 20.802 | 0.00003 | 0.261 | 0.248 | |
| Interleukin 17 | IL-17 | 1 | 0.078697 | 0.078697 | 11.834 | 0.00129 | 0.212 | 0.194 | |
| Immunoglobulin M | IGM | 1 | 2.155 | 2.15496 | 21.669 | 0.00001 | 0.192 | 0.184 | |
| Interleukin 8 | IL8 | 1 | 0.7799 | 0.77992 | 13.9 | 0.00042 | 0.183 | 0.170 | |
| Transforming growth factor beta | TGFB | 1 | 0.37021 | 0.37021 | 10.454 | 0.00201 | 0.151 | 0.136 | |
| Interleulin 1 beta | IL-1B | 1 | 0.4017 | 0.40172 | 3.5993 | 0.06270 | 0.058 | 0.042 | |
| Cluster of differentiation 4 | CD4 | 1 | 0.1036 | 0.103601 | 2.2298 | 0.13920 | 0.026 | 0.015 | |
| STAT1 | STAT1 | 1 | 0.1424 | 0.14238 | 0.7875 | 0.37850 | 0.013 | −0.004 | |
| Interferon alpha | IFNA | 1 | 0.0231 | 0.023098 | 0.7709 | 0.38280 | 0.010 | −0.003 | |
| Type I histocompatibility complex | MHCI | 1 | 0.0691 | 0.069117 | 0.6599 | 0.41920 | 0.009 | −0.005 | |
| Type II histocompatibility complex | MHCII | 1 | 0.00711 | 0.007109 | 0.1855 | 0.66800 | 0.003 | −0.011 |
In black, biomarkers that showed a significant degree of association (p < 0.0001; R2 > 0,3) with the abundance of msa transcription of the bacterium in Log10 in kidney samples (Df, degree of freedom; SS, sum of squares; MS, mean squares).