Table 1.
Identification of Mtb genes associated with formation of protective lymphoid follicles: Transposon site hybridization library (TraSH) using the himar1 transposon mutagenesis strategy was employed to create a Mtb mutant library for the specific discovery of Mtb genes influencing the progression of lymphoid follicle containing granulomas in NHPs.
| Mtb genes identified | Putative function |
|---|---|
| from NHP transposon screen | |
| mmpL2 (Rv0507) 106 kDa | Fatty acid transport |
| mmpL7 (Rv2942) 95 kDa | Fatty acid transport |
| ndhA (Rv0392c) 50 kDa | Electron transport |
| eccD5 (Rv1795) 53 kDa | Type VII secretion (*) |
| clpB (Rv0384c) | Molecular chaperone (*) |
| acr2 (Rv0251c) | Molecular chaperone |
| glnA2 (Rv2222c) | Glutamine biosynthesis |
| glnA4 (Rv2860c) | Glutamine biosynthesis |
| cmtR (Rv1994c) | Metal sensor, transcriptional regulator |
NHPs were infected with 1 × 105 CFU of Mtb H37Rv mutants and lung sections were harvested 4–6 weeks after infection. In combination with mesodissection, the analysis of >1,200 lesions identified 9 Mtb mutants that were highly represented within protective iBALT containing TB granulomas in macaques.
*
Essential gene.