Table 1.

Determination of LLoD (in mg/dL), Based on the SD of the Lowest Total Area Fragment Ion Detected in Each Dilution Curve and on the Slope Calculated by Quasar,^17,18 in Skyline,¹³ and Increase in Sensitivity

Protein	Peptide	Before enrichment, mg/dL	After enrichment, mg/dL	Increase in sensitivity, fold change
κ
κ	SGTASVVCLLNNFYPR	0.16	0.04	4.6
	VDNALQSGNSQESVTEQDSK	0.23	0.15	1.5
IgG	NQVSLTCLVK	0.29	0.02	12.8
SILuMAb Only	GLEWVSK	0.21	0.02	14.1
λ
λ	AAPSVTLFPPSSEELQANK	0.07	0.05	1.5
	YAASSYLSLTPEQWK	0.05	0.01	6.3
IgG	NQVSLTCLVK	0.47	0.03	15.2
SILuMAb Only	GLEWVSK	0.09	0.01	8.7

LLoD, lower limit of detection.