Table 1.
Primers used in Qrt-PCR
| Gene name | Annotation in gene database | Forward (FP) and reverse primers (RP) | Product size (bp) |
|---|---|---|---|
| Up-regulated DEGs | |||
| EGR_03608 | Cornifelin | FP: AGTCTGCTTCCATTGACGACCA | 149 |
| RP: CCATCGCTGTCGCCTTCGGTA | |||
| EGR_00384 | Thioredoxin-related transmembrane protein | FP: TGCAAATGTACGCTACTACCC | 88 |
| RP: ACGTAAAGTAGGCTCAAGCAA | |||
| EGR_01086 | Vesicle transport through interaction with t-SNAREs 1A | FP: AGATAGCAGCAACTACCCGTTT | 91 |
| RP: TTCACATCAGCAGGCATGTCC | |||
| EGR_06269 | Minor histocompatibility H13 | FP: TATTACCGTTTTCCGTCGAGA | 96 |
| RP: GCGGCAAATATCTTAAACACC | |||
| EGR_09743 | Collagen, type XV, alpha | FP: AAAGGACTCTACTGGACCTCG | 84 |
| RP: GAAGTCCACTGATCGCACGTT | |||
| EGR_08242 | Signal transducing adapter molecule | FP: TGATGCCCAAAATGCTACACC | 140 |
| RP: CGAAATCGAATAGAGCACGAAC | |||
|
| |||
| Down-regulated DEGs | |||
| EGR_10426 | Transcription elongation factor | FP: TGATTGAGCCACTACCGAAGCA | 123 |
| RP: AGTCCTCCAGGCATATTCCAC | |||
| EGR_08627 | Fatty acid-binding protein | FP: AAACCCGTGCTTACCTTCG | 145 |
| RP: CATCCGTGGTGTTCTCATCG | |||
| EGR_09751 | heat shock protein | FP: TCCAGGCCGTATTACTGAGCA | 96 |
| RP: ATGACTTTAGTGCCACCGT | |||
| EGR_09229 | LIM and SH3 domain protein | FP: GCCAGTGTGACGTAGTCCTC | 107 |
| RP: CGTTTGTGTGTTCTTCGCCAA | |||
| EGR_00894 | Hypothetical protein | FP: AGTCAATTCAAGTAATCGTGCTG | 80 |
| RP: TTCTCGCTTGATTTTCGCCACT | |||
| EGR_03601 | BoIA-like protein 3 | FP: TTCTTAATGTCTGCCGCCAT | 104 |
| RP: AGTAGAGCCTCCTTATAGTCC | |||
|
| |||
| Control primer | |||
| EGR_09068 | Actin II | FP: ATGGTTGGTATGGGACAAAAGG | 118 |
| RP: TTCGTCACAATACCGTGCTC | |||