Fig. 2.
TROY interacts with JAK1 through its cytoplasmic domain. (A) Schematic representation of the expression constructs encoding JAK1, TROY WT, and TROY variants. Φ-KD, pseudokinase domain; KD, Kinase domain; αv, integrin αv cytoplasmic domain; TRAFm, mutation of TRAF binding domain. The extracellular domain, the transmembrane (TM) domain, and cytoplasmic domains of TROY are indicated and the 3X HA epitope tag is shown. (B) HEK293 cells were co-transfected with FLAG epitope-tagged JAK1 and the indicated TROY construct, lysed 24 h after transfection, and immunoprecipitated with an anti-HA antibody. Immunoprecipitates or whole cell lysates (WCL) were IB with the indicated antibodies. (C) HEK293 cells were co-transfected with FLAG epitope-tagged JAK1 and the indicated TROY construct, lysed 24 h after transfection, and immunoprecipitated with an anti-HA antibody. Immunoprecipitates or WCL were IB with the indicated antibodies.