Fig. 4.
Increased TROY expression activates JAK1/STAT3 pathway. (A) Immunoblots of lysates of T98G cells transfected with increasing amounts of TROY plasmid. Cells were transfected with HA-tagged TROY plasmid for 24 h followed by overnight serum starvation. The values for p/t STAT3 represent the ratio of phosphorylated STAT3 to total STAT3 and were normalized to GAPDH. (B) T98G and T98G/TROY-HA cells were transfected with siRNA targeting JAK1 (siJAK1) or control siRNA (siCtrl). Forty-eight hours after transfection the cells were serum starved overnight, lysed, and immunoblotted with the indicated antibodies. (C) A cell based STAT3 reporter assay indicated STAT3 activation following expression of TROY and TROY-TRAFm variant but not the TROY-αv variant. Immunoblots for these samples show the equivalent expression of TROY and its variants. ***, p < 0.001.