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. Author manuscript; available in PMC: 2020 Jul 7.
Published in final edited form as: J Am Chem Soc. 2020 Apr 22;142(18):8403–8411. doi: 10.1021/jacs.0c02036

Figure 2.

Figure 2.

Essential dynamics as extracted from PCA of the combined 3-replicas pseudotrajectory. Red and green arrows depict the type and the direction of the motions. (A) Principal Component 1. Clf1 (orange) and RNase-H domain (lilac) are the arms of the hammer-like movement toward Cwc2 (green). The inset captures the wrapping of the intron-lariat (IL)/U2 double helix promoted by the β-finger motif of the RNase-H domain. (B) Principal Component 2. Clf1 and RNase-H domain perform a downward rotation. U2 and U6 snRNA are shown as orange and blue new cartoons, respectively. The inset focuses on the rotation of the RNase-H domain toward Cwc25 (dark blue). Prp8 and its RNase-H are depicted as light blue surface and lilac new cartoons, respectively. IL, Cwc2, Cwc25, Cef1, Clf1, Prp46, and U2 and U6 snRNA are shown as yellow, green, dark blue, pink, orange, light green, orange, and blue new cartoons, respectively.