Subject | Environmental Chemistry |
Specific subject area | Analytical chemistry applied to biological samples to perform biomonitoring of environmental pollutants |
Type of data | Tables and figures |
How data were acquired | Ultra-high performance liquid chromatography tandem coupled to triple quadrupole mass spectrometry (LC-MS/MS). Agilent Technologies (Palo Alto, USA) models 1290 (UHPLC) and 6460 (MS/MS). Gas chromatography tandem coupled to triple quadrupole mass spectrometry (GC-MS/MS). Agilent Technologies (Palo Alto, USA) models 7890B (GC) and 7010 (MS/MS). |
Data format | Analyzed: • Extracted and analyzed LC-MS/MS and GC-MS/MS data for the validation studies • Quantified chromatogram data of pollutants in the blood of barn owls and common kestrels Raw data: • All raw data corresponding to the 5 replicates of each concentration tested in the validation experiments presented in Table 1. • All individual quantitative data obtained after application of the method on 148 common kestrels and 39 barn owls presented in Tables 2–5. • All raw data used for the elaboration of Fig. 1. |
Parameters for data collection | The validation data of the developed method included, limit of quantification (as the lowest calibrator that fulfilled de validation criteria), linearity, accuracy (expressed as % bias and precision (intra- and inter-day RSDs)) for each of the 360 analytes. The matrix effect on the quantification of the analytes is graphically represented as percentages. For clarity the results are expressed as relative percentage. The quantification data were obtained analyzing a series of 148 blood samples, obtained from a field ecology work in nest boxes of barn owls (Tyto alba, n = 36), and common kestrels (Falco tinnunculus, n = 112). |
Description of data collection | MassHunter Quantitative Analysis was employed to collect and analyze the chromatographic data delivered by the triple quadrupole mass spectrometers coupled to both UHPLC and GC. The linearity was assessed by injecting a 12-point calibration curve prepared in the blank matrix and extracted with the developed micro-QuEChERS method. To test bias and precision, standard solutions of 360 reference standards were employed to spike blank whole blood samples at five concentration levels (0.1, 0.5, 1, 5, and 20 ng/ml) were injected in quintuplicate. The bias and repeatability (intra-day variability) were determined by those quintuplicate analyses of each sample, as these were injected within 24 h. The reproducibility (inter-day variability) was measured on three non-consecutive days within a two-week span. The matrix effect was assessed by extracting enough amount of blank matrix with the developed method and fortifying these extracts with three levels of the mixture of 360 chemicals (0.2, 2, and 10 ng/ml), and quantified against a calibration curve prepared in the solvent (1% FA-acetonitrile). Regarding the quantitative data, the real samples were prepared with the developed methodology and analyzed by UHPLC and GC. |
Data source location | Institution: Toxicology Unit, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria City/Town/Region: Las Palmas de Gran Canaria Country: Spain |
Data accessibility | With the article. Raw data are provided |
Related research article | Rial-Berriel, C., Acosta-Dacal, A., Zumbado, M., Luzardo, O.P. Micro QuEChERS-based methodology for the simultaneous biomonitoring in whole blood of 360 toxicologically relevant pollutants for wildlife. Science of the Total Environment 736 (2020) 13944 |