Skip to main content
. 2020 Jul 4;6(7):e04263. doi: 10.1016/j.heliyon.2020.e04263

Figure 4.

Figure 4

The relative level of integrated fluorescence induced under optimal immobilization conditions. A. HIF-1::GFP. Hypoxia was used as a positive control. Each sample was imaged immediately after exposure due to the transient nature of hif-1 signaling [40]. B. TMEM-135::GFP. Cold shock was used as the positive control. Each sample was imaged immediately after exposure. The inset shows CS (+) [2hr cold shock] vs CS (w/o) without room temperature post immobilization exposure. C. HSP-4::GFP. Each was allowed to recover for 24 h at 20 °C prior to imaging. D. hsp-16.2p::GFP. Heat shock was used as a positive control. Each was allowed to recover for 1 h at 20 °C prior to imaging. E. gcs-1p::GFP. Heat shock was used as a positive control. Each sample was allowed to recover for 4 h at 20 °C prior to imaging. (∗∗∗) denotes a two-tailed Student's t-test p-value < 0.05 and n = 25 was used in all assays.