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BPA decreases urogenital sinus epithelium thickness. To calculate the urothelium thickness (UET), two distances were measured on both the left and right lateral sides of the urothelium at the widest point of the urethra and averaged (see text for details; A, B). Drawn red line indicates area of urothelium, as defined by tissue positive for p63 protein via IHC. At the widest section (C) and midway section (D), the urothelium thickness was analyzed by treatment.
