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. 2020 Jun 30;11:1151. doi: 10.3389/fimmu.2020.01151

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Copyright © 2020 Mayer, Preuss, Grottenthaler, Berg and Klein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SDS-gel electrophoresis and Western blotting for the demonstration of proteins in the immunoglobulin fractions isolated by Melon IgG Spin purification kit and ammonium sulfate (AS) precipitation from a serum of a healthy donor. (A) Coomassie staining, (B–D) Western blotting with anti-human HRP-conjugated antibodies: (B) anti-human IgG, (C) anti-human IgM, (D) anti-human IgA antibodies; M, molecular weight marker; lane 1, immunoglobulin purified from serum using Melon IgG purification kit; lane 2, AS precipitated proteins. In both fractions only proteins related to immunoglobulins can be observed.