TABLE 2.
Regulation of ion channel functioning by different alkaloids: GSC – the conductance of single SyrE- or GrA-channels in the absence and presence of 0.4 mM alkaloid at V = 200 mV; τ – the dwell time of SyrE or GrA channels; I∞/I∞0 – the ratio of the transmembrane current induced by the CecA or Nys in the presence and in the absence of alkaloids at V = 50 mV.
| Alkaloid |
POPC |
POPC |
DOPE/DOPS |
POPC/Chol |
||
|
SyrE |
GrA |
CecA |
Nys |
|||
| GSC, pS | τ, ms | GSC, pS | τ, s | I∞/I∞0 | I∞/I∞0 | |
| Control | 49 ± 1 | 114 ± 4 | 22.5 ± 0.6 | 3.0 ± 1.0 | – | – |
| Pentoxifylline | 50 ± 2 | 140 ± 9 | 23.0 ± 0.8 | 3.5 ± 0.9 | 0.9 ± 0.1 | 1.0 ± 0.1 |
| Piperine | 47 ± 2 | 5 ± 1 | 22.0 ± 1.1 | 2.7 ± 1.1 | 0.3 ± 0.1 | 4.5 ± 0.9 |
| Capsaicin | 45 ± 3 | 1 ± 1 | 25.0 ± 1.0 | 5.9 ± 2.0 | 0.1 ± 0.1 | 4.1 ± 1.0 |
| Dihydrocapsaicin | 46 ± 2 | 1 ± 1 | 26.6 ± 0.8 | 4.8 ± 1.0 | 0.2 ± 0.1 | 9.8 ± 2.3 |
| Synephrine | 49 ± 2 | 67 ± 2 | 22.0 ± 0.8 | 2.8 ± 1.1 | 1.1 ± 0.4 | 1.2 ± 0.1 |
The lipid composition of bilayers is presented in this table. The membranes modified by SyrE, GrA, CecA, and Nys were bathed in 2.0, 1.0, 0.1, and 2.0 M KCl, respectively (pH 7.4).