Figure 1.

PCDH17 is up‐regulated in CH in vivo and in vitro and its silence alleviates Ang‐II‐induced CH. A, qRT‐PCR of mRNA level and Western blots of protein level of hypertrophic biomarkers TAC mouse heart versus sham control. B, qRT‐PCR of PCDH17 level in TAC mouse heart versus sham control. C, Immunofluorescence staining picture and quantification of cell surface area of Ang‐II‐induced cardiomyocytes (PCM and H9c2) versus control. D‐E, qRT‐PCR data and Western blots of the levels of hypertrophic biomarkers in cardiomyocytes under  Ang‐II treatment. F, qRT‐PCR detected PCDH17 expression in cardiomyocytes. G. The depletion efficiency of PCDH17 in Ang‐II‐induced cardiomyocytes was confirmed by qRT‐PCR. H, Immunofluorescence staining assay detected cell surface area of Ang‐II‐induced cardiomyocytes transfected with sh‐PCDH17#1/2. I‐J, qRT‐PCR and Western blot of levels of hypertrophic biomarkers in Ang‐II‐induced cardiomyocytes transfected with sh‐PCDH17#1/2. ^** P < .01