FIGURE 5.

Effects of EGF on the expression levels of steroidogenesis‐related genes in rat immature Leydig cells. After rat progenitor Leydig cells (PLCs) were cultured with 0, 1 and 10 ng/mL EGF as well as 1 ng/mL LH for 24 h, cells were subjected to qPCR analysis. The expression levels of steroidogenesis‐related genes were measured and calculated adjusted to Rps16, the internal control. Mean ± SEM, n = 4; identical letters indicate that there is no significant difference between two groups at P < .05