TABLE 1.
Genes were up‐regulated after EGF treatment
| Gene | Gene name | Expression levels | ||||
|---|---|---|---|---|---|---|
| EGF (ng/mL) | LH (ng/mL) | |||||
| 0 | 1 | 10 | 1 | |||
| Cell cycle regulation/ DNA repair | ||||||
| Ercc1 | Endonuclease non‐catalytic subunit | 641 ± 35 | 1138 ± 30 (1.8) | 1750 ± 115 (+2.7) | 951 ± 41 (+1.5) | |
| Ccnd1 | Cyclin D1 | 85 ± 3 | 120 ± 2 (+1.4) | 219 ± 1 (+2.6) | 93 ± 3 (+1.1) | |
| Phlda11 | Pleckstrin homology like domain A | 246 ± 11 | 438 ± 17 (+1.8) | 621 ± 3 (+2.5) | 528 ± 2 (+2.2) | |
| Reln | Reelin | 415 ± 6 | 643 ± 26 (+1.5) | 1038 ± 43 (+2.5) | 819 ± 29 (+2.0) | |
| Schip1 | Schwannomin interacting protein 1 | 140 ± 2 | 245 ± 8 (+1.8) | 319 ± 6 (+2.3) | 211 ± 5 (+0.6) | |
| Cd82 | Metastasis suppressor kangai‐1 | 478 ± 6 | 785 ± 14 (+1.6) | 1070 ± 28 (+2.2) | 754 ± 16 (+1.6) | |
| Cell metabolism | ||||||
| Upp1 | Uridine phosphorylase 1 | 1006 ± 54 | 3816 ± 41 (+3.8) | 5182 ± 99 (+5.1) | 2897 ± 38 (+2.8) | |
| Car2 | Carbonic anhydrase II | 250 ± 3 | 721 ± 29 (+2.9) | 1093 ± 72 (+4.4) | 1033 ± 20 (+4.2) | |
| Hs3st1 | heparan sulfate‐glucosamine 3‐sulfotransferase 1 | 146 ± 14 | 393 ± 5 (+2.7) | 577 ± 14 (+4.0) | 275 ± 8 (+2.0) | |
| Dusp6 | Dual specificity phosphatase 6 | 1150 ± 25 | 3183 ± 195 (+2.8) | 4408 ± 81 (+3.8) | 202 848± (+1.9) | |
| Aadat | Aminoadipate aminotransferase | 304 ± 4 | 763 ± 31 (+2.5) | 971 ± 15 (+3.2) | 1963 ± 36 (+6.2) | |
| Smpd1 | Sphingomyelin phosphodiesterase 1 | 6626 ± 242 | 14 384± (+2.2) | 18 775± (+2.8) | 12 677 ± 807 (+2.0) | |
| Plscr1 | Phospholipid scramblase 1 | 166 ± 6 | 280 ± 25 (+1.7) | 423 ± 28 (+2.6) | 459 ± 4 (2.7) | |
| Lipg | Endothelial lipase | 145 ± 1 | 254 ± 13 (+1.8) | 337 ± 24 (+2.3) | 201 ± 3 (+0.6) | |
| Ligand/receptors | ||||||
| Adm | Adrenomedullin | 1662 ± 46 | 3557 ± 71 (+2.1) | 4828 ± 68 (+2.9) | 2131 ± 51 (+1.2) | |
| Gal | Galanin | 542 ± 23 | 1229 ± 48 (+2.3) | 1524 ± 100 (+2.8) | 558 ± 28 (+1.1) | |
| Gchfr | GTP cyclohydrolase I | 175 ± 8 | 347 ± 3 (+2.0) | 465 ± 12 (+2.7) | 150 ± 10 (+1.0) | |
| Serpine1 | Plasminogen activator inhibitor 1 | 805 ± 41 | 1819 ± 66 (+2.3) | 2124 ± 29 (+2.6) | 1482 ± 29 (+1.6) | |
| Plcd4 | Phospholipase C delta 4 | 422 ± 13 | 1014 ± 23 (+2.4) | 1081 ± 24 (+2.6) | 447 ± 24 (+1.0) | |
| Tnfrsf12a | TNF receptor superfamily 12A | 1230 ± 17 | 2237 ± 88 (+1.8) | 3146 ± 120 (+2.6) | 2189 ± 69 (+1.6) | |
| Cxcr4 | C‐X‐C motif chemokine receptor 4 | 253 ± 9 | 491 ± 19 (+1.9) | 626 ± 11 (+2.5) | 444 ± 23 (+1.6) | |
| Mmp10 | Matrix metalloproteinases 10 | 3312 ± 113 | 5671 ± 135 (+1.7) | 12 386 ± 255 (+3.7) | 4322 ± 103 (+1.3) | |
| Alcam | CD166 antigen | 560 ± 7 | 971 ± 27 (+1.7) | 1367 ± 51 (+2.4) | 641 ± 25 (+1.3) | |
| Procr | Protein C receptor | 466 ± 17 | 712 ± 10 (+1.5) | 1097 ± 96 (+2.4) | 1088 ± 38 (+2.0) | |
| Ednrb | Endothelin receptor type B | 2304 ± 73 | 4360 ± 61 (+1.9) | 5238 ± 232 (+2.3) | 7117 ± 278 (+3.0) | |
| Areg | Amphiregulin | 238 ± 9 | 385 ± 18 (+1.6) | 488 ± 4 (+2.2) | 406 ± 22 (+1.8) | |
| Pdgfa | Platelet‐derived growth factor A | 1734 ± 37 | 2475 ± 84 (+1.4) | 3736 ± 43 (+2.2) | 2530 ± 81 (+1.4) | |
| Stc1 | Stanniocalcin 1 | 1818 ± 86 | 2899 ± 92 (+1.6) | 3809 ± 110 (+2.1) | 3773 ± 265 (+2.0) | |
| Transcription factor activity | ||||||
| Maf | Proto‐oncogene c‐Maf | 697 ± 17 | 2198 ± 68 (+3.2) | 2914 ± 55 (+4.2) | 1420 ± 15 (+2.2) | |
| Hmga1 | High mobility group AT‐hook 1 | 370 ± 11 | 812 ± 1 (+2.2) | 1011 ± 57 (+2.7) | 662 ± 38 (+1.8) | |
| Ehd4 | EH domain containing 4 | 459 ± 17 | 820 ± 50 (+1.8) | 1123 ± 62 (+2.4) | 710 ± 29 (+1.6) | |
| Runx1 | Runt related transcription factor 1 | 607 ± 25 | 1276 ± 40 (+2.1) | 1476 ± 59 (+2.4) | 1542 ± 65 (+2.5) | |
| Arhgap22 | Rho GTPase activating protein 22 | 148 ± 4 | 259 ± 1 (+1.7) | 349 ± 10 (+2.4) | 194 ± 16 (+1.3) | |
| Abhd3 | Abhydrolase domain containing 3 | 980 ± 34 | 1876 ± 60 (+1.9) | 2092 ± 164 (+2.1) | 1608 ± 56 (+1.6) | |
| Twist1 | Twist BHLH transcription factor | 275 ± 13 | 464 ± 8 (+1.7) | 587 ± 46 (+2.1) | 434 ± 3 (+1.5) | |
| Transporter/Protein binding | ||||||
| Slc17a1 | solute carrier family 1 member 17a1 | 510 ± 3 | 1423 ± 27 (+2.8) | 1845 ± 70 (+3.6) | 569 ± 48 (+1.0) | |
| Slc1a5 | solute carrier family 1 member 5 | 935 ± 38 | 2153 ± 41 (+2.3) | 3114 ± 35 (+3.3) | 1663 ± 26 (+1.7) | |
| Slc16a3 | Solute carrier family 16 member 3 | 1858 ± 23 | 3451 ± 207 (+1.9) | 4645 ± 250 (+2.5) | 5015 ± 284 (+2.8) | |
| Igfbp3 | IGF‐1 binding protein 3 | 589 ± 33 | 905 ± 33 (+1.5) | 1400 ± 30 (+2.4) | 917 ± 48 (+1.2) | |
| Sh2b3 | SH2B adaptor protein 3 | 382 ± 16 | 727 ± 9 (+1.9) | 785 ± 18 (+2.2) | 520 ± 11 (+1.3) | |
| Ak3l1 | Adenylate kinase isoenzyme 4 | 462 ± 28 | 885 ± 24 (+1.9) | 1004 ± 31 (+2.2) | 1089 ± 16 (+2.1) | |
| Ube2q2 | Ubiquitin conjugating enzyme E2Q2 | 518 ± 10 | 875 ± 23 (+1.7) | 1125 ± 45 (+2.2) | 720 ± 16 (+1.4) | |
() fold up‐regulated when compared to control.