FIGURE 5.

Inhibition of NET formation by Kaempferol depends on ROS signalling. (A) Primary mouse neutrophils stimulated with PMA at 10 nmol/L to induce ROS production. Pre‐treatment of the stimulated neutrophils with kaem (100 μmol/L) or DPI (20 μmol/L) for 30 min at 37°C significantly inhibited ROS production. (B) dsRNA released by PMA‐stimulated neutrophils is inhibited by kaem or DPI, under the same condition. Data presented as mean ± SEM of three independent experiments. *P < 0.05, compared with control group; **P < 0.01, compared with control; ***P < 0.001, compared with control