FIGURE 1.

A, Rat SIRT2 (rSIRT2) coding region was cloned into pGEX‐6P‐1 expression vector. Analysis of expression and purification revealed that GST‐rSIRT2 predominantly expressed in inclusion bodies (pellet), but GST is mainly in soluble fraction (supernatant). Subsequently, both GST‐rSIRT2 and GST were purified from supernatant of the lysate. B, In a pull‐down assay, a new protein band was isolated with GST‐rSIRT2 compared with GST alone or glutathione beads. This unknown protein has an approximate molecular size of 100 kD. C, The new protein band was excised for identification by mass spectrometry, and results showed 48% sequence coverage with rat Hsp90α