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. 2020 May 29;24(13):7163–7174. doi: 10.1111/jcmm.15167

Figure 3.

Figure 3

Twist1 leading to VM formation through inhibition of Claudin15 transcription. A, The effect of Twist1 expression on VM channel formation. VM channels in 3D cell culture of MDA‐MB‐231‐shTwist1 cells and MCF‐7‐Twist1 cells. B, Wound healing assays of MDA‐MB‐231‐shTwist1 cells and MCF‐7‐Twist1 cells. C, Quantification of wound healing of MCF‐7‐Twist1 cells and MDA‐MB‐231‐shTwist1 cells (n = 3). D, Western blotting of Claudin15 expression and VE‐cadherin in the indicated cells. E, Dual‐luciferase reporter assays in MCF‐7 cells cotransfected with a Claudin15 luciferase reporter and Twist1 expression vector. F, The expression and quantifications of Twsit1, Claudin15 and VE‐cadherin in the different groups. Arrows indicated the positive signals. The ruler is 100 μm, and the error bar indicates the standard error of mean (SEM). *P < .05, **P < .01, ***P < .001