Figure 3.

Numb, HDM2 and p53 levels in the different cell fractions of the NUMB–EGFP‐transfected MDA‐MB‐231 cells. A, The protein expression of Numb, HDM2 and p53 in the membrane, cytoplasmic and nuclear fractions of NUMB‐EGFP‐transfected MDA‐MB‐231 cells was determined by Western blot. B, C, D Quantitative analysis of Numb, HDM2 and p53 proteins in different cell fractions of NUMB‐EGFP‐transfected cells. (Mean ± SD, n = 3, **P < .01, vs blank group or EGFP group). E Quantitative RT–PCR detection of NUMB, HDM2 and p53 levels in NUMB‐EGFP‐transfected MDA‐MB‐231 cells