Skip to main content
. 2020 Jun 1;10(6):1871–1887.

Figure 5.

Figure 5

miR-205 directly targeted RunX2 in breast cancer cells. A. In silico analysis of two target prediction databases showed that miR-205 targeted the 3’UTR of RunX2 mRNA, (http://mirdb.org/cgi-bin/target_detail.cgi?targetID=547892), (http://www.targetscan.org/cgi-bin/targetscan/vert_71/targetscan.cgi?species=Human&mir_sc=miR-205-5p). B. Dual luciferase reporter assays verified that RunX2 was the target gene of miR-205 (**P<0.01). C. RT-PCR result of the mRNA expression levels of miR-205 and RunX2 in different breast cell lines. D. Western blot was used to detect RunX2 expression levels after infection with miR-205 mimics in MDA-MB-231 cells and infection with miR-205 inhibitor in MCF-7 cells.