Movie 4. Cryo-SIM/FIB-SEM reveals the morphological heterogeneity of the endolysosomal system.

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A correlative data set of a SUM-159 cell after endosomal uptake of Alexa Fluor-conjugated transferrin. Part 1: 3D cryo-SIM data (green), correlative orthoslices, and correlative volume render (plasma membrane, cyan; cellular interior, orange). Part 2: ~13 μm³ sub-volume showing segmentations of all transferrin containing compartments. Part 3: same, but for a different ~20 μm³ sub-volume (Fig. 5).