Fig. 5.

Cytotoxicity of combinatorial therapy. SRB viability assays employing the mTOR inhibitor everolimus, oncolytic vaccinia virus vector GLV-1 h68 and combination of both. H727 cells originating from a lung NET and the NEC-derived NEC-DUE1 cell line were employed and analysis was performed at 96 hpi. With both cell lines, combinatorial treatment with everolimus was found to be slightly more effective than single agent treatment with either everolimus or GLV-1 h68 alone. In both cell lines and for both MOIs tested, the addition of everolimus to GLV-1 h68 further reduced the remaining tumor cell count. Experiments were carried out in quadruplicates; bars show mean and SD. * p < 0.01; ** p < 0.001